This study discusses a Madonna with St. John by Bronzino, formerly in the Aram Collection, Berlin, now in the collection of Alvan Macauley Jr., Grosse Pointe Farms, Michigan. The work is dated ca. 1540-1545 by stylistic analogy with Bronzino paintings such as the Nativity (Budapest), the Chapel of Eleonora (Florence, Palazzo Vecchio), the Madonna with St. John and St. Elizabeth (London, National Gallery), and it is suggested that it may be one of the as yet unidentified Madonnas mentioned by Vasari as painted in these years by Bronzino. It is noted that the Macauley Madonna is related to Bronzino's other Holy Families of the 1540s in its treatment of the theme of the destiny of Christ. Finally, a preparative study by Bronzino for the work is identified in Budapest, Szépmüvészeti Múzeum.